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Conditions of optimal recombinant Biotinylated Human GM-CSF R alpha Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005653: Biotinylated Human GM-CSF R alpha Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human GM-CSF R alpha Protein, (amino acids Glu23-Gly320) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human GM-CSF R alpha protein with the His-Avi tag at the C-terminus has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Anti-GM-CSF R alpha Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Human GM-CSF R alpha protein with the C-His tag with the EC50 of 11.6 ng/ml determined by ELISA (QC Test). The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human GM-CSF R alpha Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Biotinylated Human GM-CSF R alpha Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following GM-CSF R alpha Protein :
Biotinylated Human GM-CSF R alpha Protein
Cynomolgus GM-CSF R alpha Protein
Human GM-CSF R alpha Protein
Mouse GM-CSF R alpha Protein
Syd Labs provides the following GM-CSF Protein :
GM-CSF Protein
Human GM-CSF Protein
Mouse GM-CSF Protein
Syd Labs provides the following GM-CSF Antibody :
GM-CSF Antibody
Syd Labs provides the following GM-CSF ELISA Kit :
Mouse GM-CSF ELISA Kit
Rat GM-CSF ELISA Kit
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